ANF MetaBioDiv

Forth edition (September 2026)

Welcome to the third edition of the ANF Exploration de la Diversité Taxonomique des Ecosystèmes par Metabarcoding. The aim of this course is to teach you how to run microbial ecology analyses (mostly alpha and beta diversity) from metabarcoding raw sequencing data using R.

Program

You can also find a pdf version of the program here

• Monday 28^th:
  • 09h00: Participants arrival and installation
  • 12h30:
  • 14h00: Training introduction I
  • 14h15: Introduction and R practical by Marc Garel + check technical issues
  • 16h00:
  • 16h15: R practical by Marc Garel + check technical issues
  • 18h00: Seminar “Introduction séquençage long read ONT” by Fabrice Armougom
  • 18h45: Welcoming drink
  • 19h30: Diner
• Tuesday 29^th:
  • 08h45: Introduction Metabarcoding to metabarcoding by Loïs Maignien
  • 09h30: Analysing metabarcoding data with dada2 by Charlotte Berthelier/Nicolas Henry
  • 10h30:
  • 10h45: Preprocessing: dada2 by Charlotte Berthelier/
  • 12h30:
  • 14h00: Introduction to phyloseq
  • 15h15:
  • 15h30: Séminaire metabarcoding worklows by Charlotte Berthelier/Nicolas Henry
  • 16h30: Ampliseq configuration workflow by Charlotte Berthelier and Nicolas Henry
  • 18h00: Set up personal data backups for those who wish to do so in their free time
  • 19h30:
• Wednesday 30^st:
  • 08h45: Course on ONT long read metabarcoding by Fabrice Armougom
  • 09:30: Demo preprocessing ONT long read metabarcoding by Fabrice Armougom/Elsa Mendes
  • 10h30:
  • 10h45: Output analysis to determine quality score by Fabrice Armougom/Elsa Mendes
  • 12h30:
  • 13h45: Course on alphadiversity and statistics by Elsa Mendes
  • 14h15: Alpha diversity practical by Elsa Mendes/Marc Garel
  • 18h00: Set up personal data backups for those who wish to do so in their free time
  • 19h30:
• Thursday 1^st:
  • 08h45: Course on beta diversity 1/2 by Jean-Christophe Auguet
  • 10h15:
  • 10h30: Beta diversity practical by Jean-Christophe Auguet
  • 12h30:
  • 14h00: Course on beta diversity 2/2 by Jean-Christophe Auguet
  • 15h00: Beta diversity practical by Jean-Christophe Auguet
  • 16h00:
  • 16h15: Beta diversity practical by Jean-Christophe Auguet
  • 18h00: free time
  • 19h30:
• Friday 2^nd:
  • 09h00: À la carte with
  • 12h30:
  • 14h00: End of the course